| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87772-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Cy3 | NB120-11319 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87771-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35916-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60245-50ul | |||
![Western Blot: gamma Tubulin Antibody (TU-30) [NB500-574] - Western blotting analysis of differential reactivity of monoclonal antibodies to gamma-tubulin with human gamma-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human gamma-tubulin 1 (gamma-Tb1) or gamma-tubulin 2 (gamma-Tb2), probed with Abs to gamma-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of gamma-Tb1 or gamma-Tb2 probed with Abs to gamma-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-TU-30-Western-Blot-NB500-574-img0005.jpg "Western Blot: gamma Tubulin Antibody (TU-30) [NB500-574] - Western blotting analysis of differential reactivity of monoclonal antibodies to gamma-tubulin with human gamma-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human gamma-tubulin 1 (gamma-Tb1) or gamma-tubulin 2 (gamma-Tb2), probed with Abs to gamma-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of gamma-Tb1 or gamma-Tb2 probed with Abs to gamma-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.")
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (TU-30) [NB500-574] - Staining of HeLa cells using anti-gamma Tubulin antibody (green). Nuclei were stained with DAPI (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-TU-30-Immunofluorescence-NB500-574-img0004.jpg)
![Immunohistochemistry-Paraffin: gamma Tubulin Antibody (TU-30) - BSA Free [NB500-574] - FFPE Glioblastoma PDX is stained with mouse-gamma tubulin antibody (NB500-574) (red) for centrosomes and rabbit Arl13B (green) for primary cilia. Gamma Tubulin antibody is diluted 1:25, incubated overnight at 4C. Image from verified customer review.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-TU-30-BSA-Free-Immunohistochemistry-Paraffin-NB500-574-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (TU-30) [NB500-574] - Staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue).](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-TU-30-Immunocytochemistry-Immunofluorescence-NB500-574-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (TU-30) [NB500-574] - Staining of mouse fibroblasts using anti-gamma-tubulin (TU-30; direct conjugate with Dyomics 547, red). Nuclei were stained with DAPI (blue).](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-TU-30-Immunocytochemistry-Immunofluorescence-NB500-574-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (TU-30) [NB500-574] - Staining of microtubular networks in 3T3 mouse fibroblasts. 3A - metaphase; 3B - anaphase; 3C - telophase Gamma-tubulin (red) stained with anti-gamma-tubulin (TU-30), alpha-tubulin (green) with polyclonal anti-alpha-tubulin antibody and nuclei with DAPI (blue).](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-TU-30-Immunocytochemistry-Immunofluorescence-NB500-574-img0003.jpg)
![Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Analysis in human testis and liver tissues. Corresponding Pericentrin RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunohistochemistry-Paraffin-NBP1-87772-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Analysis in human testis and liver tissues. Corresponding Pericentrin RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human cerebral cortex, liver, placenta and testis using Anti-Pericentrin antibody NBP1-87772 (A) shows similar protein distribution across tissues to independent antibody NBP1-87771 (B).](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunohistochemistry-Paraffin-NBP1-87772-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Pericentrin Antibody [NBP1-87772] - Smear of -20C methanol-fixed mouse small intestinal epithelial cells stained for ZO-1 (red) and pericentrin (green). ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87772-img0019.jpg)
![Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunohistochemistry-Paraffin-NBP1-87772-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Pericentrin Antibody [NBP1-87772] - Staining of human cell line A-431 shows localization to centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87772-img0006.jpg)
![Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunohistochemistry-Paraffin-NBP1-87772-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining in human testis and liver tissues using anti-PCNT antibody. Corresponding PCNT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunohistochemistry-Paraffin-NBP1-87772-img0010.jpg)
![Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunohistochemistry-Paraffin-NBP1-87772-img0015.jpg)
![Immunohistochemistry: Pericentrin Antibody [NB100-61071] - Immunofluorescent analysis of cilia in renal tissues. Sections (4 um) of renal parenchymal tissues and tumor tissues were stained with DAPI, acetylated-alpha-tubulin (Ac-tub) and pericentrin (PCNT) to mark cell nuclei and cilia. Presented images are maximal projections of confocal images of typical parenchymal tissue and a representative ccRCC. Scale bars 20 um. Statistics were determined by performing a paired t-test at a 95% confidence interval. Image collected and cropped by CiteAb from the following publication (//ciliajournal.biomedcentral.com/articles/10.1186/2046-2530-2-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunohistochemistry-NB100-61071-img0016.jpg "Immunohistochemistry: Pericentrin Antibody [NB100-61071] - Immunofluorescent analysis of cilia in renal tissues. Sections (4 um) of renal parenchymal tissues and tumor tissues were stained with DAPI, acetylated-alpha-tubulin (Ac-tub) and pericentrin (PCNT) to mark cell nuclei and cilia. Presented images are maximal projections of confocal images of typical parenchymal tissue and a representative ccRCC. Scale bars 20 um. Statistics were determined by performing a paired t-test at a 95% confidence interval. Image collected and cropped by CiteAb from the following publication (//ciliajournal.biomedcentral.com/articles/10.1186/2046-2530-2-2) licensed under a CC-BY license.")
![Western Blot: Pericentrin Antibody [NB100-61071] - Whole cell lysate (10 ug) from HEK293T, HeLa, Hep-G2, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pericentrin-Kendrinantibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 second.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Western-Blot-NB100-61071-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Pericentrin Antibody [NB100-61071] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Pericentrin/Kendrin used at a dilution of 1:1,000 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight (R) 594 Conjugated used at a dilution of 1:100. Counterstain. DAPI (blue) and Phalloidin (green)](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-61071-img0014.jpg)
![Immunoprecipitation: Pericentrin Antibody [NB100-61071] - Detection of human Pericentrin/Kendrin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pericentrin/Kendrin antibody NB100-61071 (lot 3) used for IP at 6 ug per reaction. Pericentrin/Kendrin was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated Pericentrin/Kendrin, NB100-61071 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunoprecipitation-NB100-61071-img0015.jpg)
![Western Blot: gamma Tubulin Antibody (5R3N3) [NBP3-16852] - Western blot analysis of extracts of various cell lines, using gamma Tubulin Rabbit mAb (NBP3-16852) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-5R3N3-Western-Blot-NBP3-16852-img0007.jpg "Western Blot: gamma Tubulin Antibody (5R3N3) [NBP3-16852] - Western blot analysis of extracts of various cell lines, using gamma Tubulin Rabbit mAb (NBP3-16852) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunohistochemistry-Paraffin: gamma Tubulin Antibody (5R3N3) [NBP3-16852] - Immunohistochemistry of paraffin-embedded mouse testis using gamma Tubulin Rabbit mAb (NBP3-16852) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-5R3N3-Immunohistochemistry-Paraffin-NBP3-16852-img0003.jpg)
![Immunohistochemistry-Paraffin: gamma Tubulin Antibody (5R3N3) [NBP3-16852] - Immunohistochemistry of paraffin-embedded rat testis using gamma Tubulin Rabbit mAb (NBP3-16852) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-5R3N3-Immunohistochemistry-Paraffin-NBP3-16852-img0001.jpg)
![Immunohistochemistry-Paraffin: gamma Tubulin Antibody (5R3N3) [NBP3-16852] - Immunohistochemistry of paraffin-embedded human thyroid cancer using gamma Tubulin Rabbit mAb (NBP3-16852) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-5R3N3-Immunohistochemistry-Paraffin-NBP3-16852-img0002.jpg)
![Western Blot: gamma Tubulin Antibody (5R3N3) [NBP3-16852] - Western Blot of the lysate of RM1 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp3-16852_rabbit-gamma-tubulin-mab-5r3n3-western-blot-672023121354..jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (5R3N3) [NBP3-16852] - Confocal imaging of NIH-3T3 cells using gamma -Tubulin Rabbit mAb (dilution 1:100) (Red). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit mAb (dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.](http://images.novusbio.com/fullsize/nbp3-16852_rabbit-gamma-tubulin-mab-5r3n3-immunocytochemistry-immunofluorescence-811202312504.jpg)
![Western Blot: Rabbit gamma Tubulin mAb (5R3N3) [NBP3-16852] - Western blot of the lysate of PC-3 cells. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp3-16852_rabbit-gamma-tubulin-mab-5r3n3-western-blot-25112024145911..png)
![Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (5R3N3) [NBP3-16852] - Confocal imaging of NIH-3T3 cells using gamma Tubulin Rabbit mAb . The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit mAb (Green). DAPI was used for nuclear staining (blue). Objective: 60x.](http://images.novusbio.com/fullsize/nbp3-16852_rabbit-gamma-tubulin-mab-5r3n3-5220251265366.jpg)
![Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (5R3N3) [NBP3-16852] - Confocal imaging of U-2OS cells using gamma Tubulin Rabbit mAb (Green). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit mAb . DAPI was used for nuclear staining (blue). Objective: 60x.](http://images.novusbio.com/fullsize/nbp3-16852_rabbit-gamma-tubulin-mab-5r3n3-52202511383724.jpg)
![Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (5R3N3) [gamma Tubulin] - Confocal imaging of NIH-3T3 cells using gamma Tubulin Rabbit mAb . The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit mAb (Green). DAPI was used for nuclear staining (blue). Objective: 60x.](http://images.novusbio.com/fullsize/nbp3-16852_rabbit-gamma-tubulin-mab-5r3n3-52202517315548.jpg)
![Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (5R3N3) [gamma Tubulin] - Confocal imaging of U-2OS cells using gamma Tubulin Rabbit mAb (Green). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit mAb . DAPI was used for nuclear staining (blue). Objective: 60x.](http://images.novusbio.com/fullsize/nbp3-16852_rabbit-gamma-tubulin-mab-5r3n3-5220251735279.jpg)
![Western Blot: gamma Tubulin Antibody (4511) [NBP2-43585] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 10 % SDS-PAGE gamma Tubulin antibody dilution: 1:500.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-4511-Western-Blot-NBP2-43585-img0004.jpg "Western Blot: gamma Tubulin Antibody (4511) [NBP2-43585] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 10 % SDS-PAGE gamma Tubulin antibody dilution: 1:500.")
![Western Blot: gamma Tubulin Antibody (4511) [NBP2-43585] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug NCI-H929 whole cell lysate/extract 10 % SDS-PAGE gamma Tubulin antibody [4511] dilution: 1:1000](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-4511-Western-Blot-NBP2-43585-img0002.jpg)
![Western Blot: gamma Tubulin Antibody (4511) [NBP2-43585] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug C8D30 whole cell lysate/extract C. 30 ug NIH-3T3 whole cell lysate/extract D. 30 ug BCL-1 whole cell lysate/extract E. 30 ug C2C12 whole cell lysate/extract 10 % SDS-PAGE gamma Tubulin antibody dilution: 1:500](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-4511-Western-Blot-NBP2-43585-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (4511) [NBP2-43585] - Analysis of HeLa cells were fixed in -20? 100% methanol for 5 min. Green: TUBG1 protein stained by TUBA1B antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-4511-Immunocytochemistry-Immunofluorescence-NBP2-43585-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (4511) [NBP2-43585] - HeLa cells were fixed in ice-cold MeOH for 5 min.Green: gamma Tubulin stained by gamma Tubulin antibody [4511] diluted at 1:500.Red: alpha Tubulin, stained by alpha Tubulin antibody diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp2-43585_mouse-monoclonal-gamma-tubulin-antibody-4511-immunocytochemistry-immunofluorescence-19122023125255..jpg)
![Western Blot: gamma Tubulin Antibody (HL1175) - Azide and BSA Free [NBP3-25476] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (NBP3-25476) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25476_rabbit-gamma-tubulin-mab-hl1175-azide-and-bsa-free-2120249413363.jpg "Western Blot: gamma Tubulin Antibody (HL1175) - Azide and BSA Free [NBP3-25476] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (NBP3-25476) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: gamma Tubulin Antibody (HL1175) - Azide and BSA Free [NBP3-25476] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (NBP3-25476) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25476_rabbit-gamma-tubulin-mab-hl1175-azide-and-bsa-free-2120249393551.jpg)
![Western Blot: gamma Tubulin Antibody (HL1175) - Azide and BSA Free [NBP3-25476] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (NBP3-25476) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25476_rabbit-gamma-tubulin-mab-hl1175-azide-and-bsa-free-2120249355526.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (HL1175) - Azide and BSA Free [NBP3-25476] - gamma Tubulin antibody [HL1175] detects gamma Tubulin protein at centrosome and mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: gamma Tubulin stained by gamma Tubulin antibody [HL1175] (NBP3-25476) diluted at 1:2500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25476_rabbit-gamma-tubulin-mab-hl1175-azide-and-bsa-free-2120249403815.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (HL1175) - Azide and BSA Free [NBP3-25476] - gamma Tubulin antibody [HL1175] detects gamma Tubulin protein at centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: gamma Tubulin stained by gamma Tubulin antibody [HL1175] (NBP3-25476) diluted at 1:2500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25476_rabbit-gamma-tubulin-mab-hl1175-azide-and-bsa-free-2120249403883.jpg)
![Western Blot: gamma Tubulin Antibody (HL1175) - Azide and BSA Free [NBP3-25476] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (NBP3-25476) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25476_rabbit-gamma-tubulin-mab-hl1175-azide-and-bsa-free-212024941436.jpg)
![Western Blot: gamma Tubulin Antibody (4D11) [NB100-74421] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing g-Tubulin at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-4D11-Western-Blot-NB100-74421-img0005.jpg "Western Blot: gamma Tubulin Antibody (4D11) [NB100-74421] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing g-Tubulin at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.")
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (4D11) [NB100-74421] - Analysis of g-Tubulin using anti-g-Tubulin monoclonal antibody (shown in green) in HeLa (A) and U2-OS cells (B).](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-4D11-Immunocytochemistry-Immunofluorescence-NB100-74421-img0006.jpg)
![Immunohistochemistry-Paraffin: gamma Tubulin Antibody (4D11) [NB100-74421] - Both normal and cancer biopsies of deparaffinized human Breast carcinoma tissue.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-4D11-Immunohistochemistry-Paraffin-NB100-74421-img0008.jpg)
![Immunohistochemistry-Paraffin: gamma Tubulin Antibody (4D11) [NB100-74421] - Both normal and cancer biopsies of deparaffinized human Colon tissue.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-4D11-Immunohistochemistry-Paraffin-NB100-74421-img0007.jpg)
![Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Expression of the mitochondrial biogenesis proteins: SIRT1, PGC1alpha, TFAM and SIRT3 in H1299, H1299r, P31 and P31r cell lysates as determined by immunoblot analysis. Whole cell lysates were prepared from confluent cultures of H1299, H1299r, P31 and P31r cells. Proteins (90 ug) were resolved in 10% SDS-PAGE gels and transferred to a PVDF membrane. Blots were probed for SIRT1, PGC1alpha, TFAM and SIRT3 or the loading control gamma-tubulin. Figure shows representative blots of (A) of SIRT1, (B) PGC1alpha, (C) SIRT3, (D) TFAM protein expression; each from three independent experiments. Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.21885) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-TU-32-Western-Blot-NB110-90616-img0004.jpg "Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Expression of the mitochondrial biogenesis proteins: SIRT1, PGC1alpha, TFAM and SIRT3 in H1299, H1299r, P31 and P31r cell lysates as determined by immunoblot analysis. Whole cell lysates were prepared from confluent cultures of H1299, H1299r, P31 and P31r cells. Proteins (90 ug) were resolved in 10% SDS-PAGE gels and transferred to a PVDF membrane. Blots were probed for SIRT1, PGC1alpha, TFAM and SIRT3 or the loading control gamma-tubulin. Figure shows representative blots of (A) of SIRT1, (B) PGC1alpha, (C) SIRT3, (D) TFAM protein expression; each from three independent experiments. Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.21885) licensed under a CC-BY license.")
![Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Analysis of gamma-Tubulin in porcine brain lysate using NBP1-90616.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-TU-32-Western-Blot-NB110-90616-img0001.jpg)
![Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Differential reactivity of monoclonal antibodies to gamma-tubulin with human gamma-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human gamma-tubulin 1 (gamma-Tb1) or gamma-tubulin 2 (gamma-Tb2), probed with Abs to gamma-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of gamma-Tb1 or gamma-Tb2 probed with Abs to gamma-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-TU-32-Western-Blot-NB110-90616-img0003.jpg)
![Western Blot: gamma Tubulin Antibody (TU-32) - BSA Free [NB110-90616] - Comparative mitochondrial abundance as measured by citrate synthase activity, abundance of VDAC1 & cytochrome oxidase subunit 4(A) Citrate synthase enzyme activity in the H1299 & H1299r cells. (B) Citrate synthase enzyme activity in the P31 & P31r cells. Data are expressed as mean ± SEM for three separate experiments. Statistical analysis was carried out using the student t-test. *** = p< 0.001, ** = p<0.01. For immunoblots, whole cell lysates were prepared from confluent cultures of H1299, H1299r, P31 & P31r cells. Proteins (90 μg) were resolved in 10% SDS-PAGE gels & transferred to a PVDF membrane. Blots were probed for either VDAC1, cytochrome c oxidase subunit 4 or the loading control gamma -tubulin. (C) Representative blot of VDAC1 protein expression from three independent experiments. (D) Representative blot of cytochrome c oxidase subunit 4 protein expression from three independent experiments. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.21885), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-90616_mouse-monoclonal-gamma-tubulin-antibody-tu-32-31020241621831.jpg)
![Western Blot: gamma Tubulin Antibody [NB120-11318] - Cell lysates (lanes 1-7) were separated on SDS-PAGE, blotted with 0.7 ug/mL Anti-gamma-Tubulin, and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. U87; 2. HeLa; 3. Jurkat; 4. C2C12; 5. Rat2; 6. MDCK; 7. A431](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-Western-Blot-NB120-11318-img0015.jpg "Western Blot: gamma Tubulin Antibody [NB120-11318] - Cell lysates (lanes 1-7) were separated on SDS-PAGE, blotted with 0.7 ug/mL Anti-gamma-Tubulin, and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. U87; 2. HeLa; 3. Jurkat; 4. C2C12; 5. Rat2; 6. MDCK; 7. A431")
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in Rat2 cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11318-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in NIH3T3 cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11318-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in NIH3T3 cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11318-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in HeLa cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11318-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Pericentrin Antibody [NBP1-87771] - Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pericentrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87771-img0004.jpg "Immunocytochemistry/Immunofluorescence: Pericentrin Antibody [NBP1-87771] - Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome. Antibody staining is shown in green.")
![Western Blot: Pericentrin Antibody [NBP3-35916] - Western blot analysis of various lysates using Pericentrin Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35916_rabbit-pericentrin-pab-912202415161820.jpg "Western Blot: Pericentrin Antibody [NBP3-35916] - Western blot analysis of various lysates using Pericentrin Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: Pericentrin Antibody [NBP3-35916] - Immunofluorescence analysis of U-2 OS cells using Pericentrin Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35916_rabbit-pericentrin-pab-9122024159538.jpg)
![Western Blot: gamma Tubulin Antibody (9D6) [NBP2-60245] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human gamma Tubulin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: Jurkat cell lysate Lane 3.: A549 cell lysate Lane 4.: K562 cell lysate](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-9D6-Western-Blot-NBP2-60245-img0001.jpg "Western Blot: gamma Tubulin Antibody (9D6) [NBP2-60245] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human gamma Tubulin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: Jurkat cell lysate Lane 3.: A549 cell lysate Lane 4.: K562 cell lysate")
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (9D6) [NBP2-60245] - Staining in HeLa cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-9D6-Immunocytochemistry-Immunofluorescence-NBP2-60245-img0002.jpg)